Study on Calcium Reinforced Polymeric Hydrogel Scaffolds for Bone Tissue Regeneration

Treatments for unwanted bone fractures have different limitations like potential infection risks and requirement of secondary surgery. Polymeric tissue engineering scaffold material can be a suitable alternative treatment device for the bone tissue regeneration due to its excellent biocompatibility, mechanical property and degradability. This thesis reports the preparation and characterization of novel calcium reinforced bacterial cellulose (BC) based hydrogel scaffolds for its possible application in bone tissue regeneration. The scaffolds were developed by using a combination of natural polymer, BC and other synthetic polymers like polyvinylpyrrolidone (PVP), polyethylene glycol (PEG). The scaffolds were prepared in two forms. First, calcium phosphate (CaP) reinforced BC based hydrogel scaffolds were prepared, where CaP was used in the form of β-tri calcium phosphate (β-TCP) and hydroxyapatite (HA) in different concentrations. Second, calcium phosphate & calcium carbonate reinforced BC based hydrogel scaffolds were prepared through template mediated in vitro biomineralization of CaP filled BC based hydrogel scaffolds. The structural properties (physiochemical, morphological, mechanical and/or viscoelastic characterization) indicated the CaP reinforced scaffolds have demonstrated significant swelling ability, suitable porosity and other mechanical and viscoelastic properties. Furthermore, functional properties (involving the biocompatibility, cell viability, cell-biomaterial interaction and bone marker expression) indicated their better efficiency for bone tissue regeneration. Thus, the CaP reinforced BC based hydrogel scaffolds (BC-PVP-β-TCP/HA_20:80 and BC-PVP-β-TCP/HA_50:50) are recommended for further analysis (e.g. in vivo study) and application in soft bone tissue (ie. cancellous bone) regeneration.

ISBN:	978-80-7454-952-6
EAN:	9788074549526
Počet stran	54 stran
Datum vydání	12. 11. 2020
Pořadí vydání	První
Jazyk	anglický
Vazba	e-kniha - pdf
Autor:	Probal Basu
Nakladatelství	Univerzita Tomáše Bati ve Zlíně
Tématická skupina	999 - nezařazeno
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